Altered Gene Regulation and Synaptic Morphology in "Drosophila" Learning and Memory Mutants

Autor/inn/en	Guan, Zhuo; Buhl, Lauren K.; Quinn, William G.; Littleton, J. Troy
Titel	Altered Gene Regulation and Synaptic Morphology in "Drosophila" Learning and Memory Mutants
Quelle	In: Learning & Memory, 18 (2011) 4, S.191-206 (16 Seiten) PDF als Volltext Verfügbarkeit
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	1072-0502
DOI	10.1101/lm.2027111
Schlagwörter	Associative Learning; Short Term Memory; Long Term Memory; Cognitive Processes; Animals; Genetics; Neurological Organization; Neurological Impairments + Suchen Sie Ihr Suchwort? Kurzzeitgedächtnis; Langzeitgedächtnis; Cognitive process; Kognitiver Prozess; Animal; Tier; Tiere; Humangenetik; Neurodegenerative Erkrankung
Abstract	Genetic studies in "Drosophila" have revealed two separable long-term memory pathways defined as anesthesia-resistant memory (ARM) and long-lasting long-term memory (LLTM). ARM is disrupted in "radish" ("rsh") mutants, whereas LLTM requires CREB-dependent protein synthesis. Although the downstream effectors of ARM and LLTM are distinct, pathways leading to these forms of memory may share the cAMP cascade critical for associative learning. "Dunce", which encodes a cAMP-specific phosphodiesterase, and "rutabaga", which encodes an adenylyl cyclase, both disrupt short-term memory. "Amnesiac" encodes a pituitary adenylyl cyclase-activating peptide homolog and is required for middle-term memory. Here, we demonstrate that the Radish protein localizes to the cytoplasm and nucleus and is a PKA phosphorylation target in vitro. To characterize how these plasticity pathways may manifest at the synaptic level, we assayed synaptic connectivity and performed an expression analysis to detect altered transcriptional networks in "rutabaga", "dunce", "amnesiac", and "radish" mutants. All four mutants disrupt specific aspects of synaptic connectivity at larval neuromuscular junctions (NMJs). Genome-wide DNA microarray analysis revealed [image omitted]375 transcripts that are altered in these mutants, suggesting defects in multiple neuronal signaling pathways. In particular, the transcriptional target "Lapsyn", which encodes a leucine-rich repeat cell adhesion protein, localizes to synapses and regulates synaptic growth. This analysis provides insights into the Radish-dependent ARM pathway and novel transcriptional targets that may contribute to memory processing in "Drosophila". (As Provided).
Anmerkungen	Cold Spring Harbor Laboratory Press. 500 Sunnyside Boulevard, Woodbury, NY 11797-2924. Tel: 800-843-4388; Tel: 516-367-8800; Fax: 516-422-4097; e-mail: cshpres@cshl.edu; Web site: http://www.learnmem.org/
Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2017/4/10