An Enzyme Kinetics Experiment for the Undergraduate Organic Chemistry Laboratory

Autor/inn/en	Olsen, Robert J.; Olsen, Julie A.; Giles, Greta A.
Titel	An Enzyme Kinetics Experiment for the Undergraduate Organic Chemistry Laboratory
Quelle	In: Journal of Chemical Education, 87 (2010) 9, S.956-957 (2 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	0021-9584
DOI	10.1021/ed100282m
Schlagwörter	Kinetics; Organic Chemistry; Science Laboratories; Biochemistry; Science Instruction; Science Experiments; College Science; Scientific Principles; Spectroscopy; Undergraduate Study + Suchen Sie Ihr Suchwort? Kinetik; Organische Chemie; Biochemie; Teaching of science; Science education; Natural sciences Lessons; Naturwissenschaftlicher Unterricht; Spektroskopie; Grundstudium
Abstract	An experiment using [superscript 1]H NMR spectroscopy to observe the kinetics of the acylase 1-catalyzed hydrolysis of "N"-acetyl-DL-methionine has been developed for the organic laboratory. The L-enantiomer of the reactant is hydrolyzed completely in less than 2 h, and [superscript 1]H NMR spectroscopic data from a single sample can be worked up to generate Michaelis-Menten and Lineweaver-Burke kinetic plots. The experiment introduces organic chemistry students to the use of a [superscript 1]H NMR spectrometer, serves as an introduction to enzyme kinetics, and illustrates the stereospecificity of enzyme catalysis. (Contains 4 figures and 3 notes.) (As Provided).
Anmerkungen	Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2017/4/10