Simple & Rapid Generation of Complex DNA Profiles for the Undergraduate Laboratory

Autor/in	Kass, David H.
Titel	Simple & Rapid Generation of Complex DNA Profiles for the Undergraduate Laboratory
Quelle	In: American Biology Teacher, 69 (2007) 3, S.163-168 (6 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	0002-7685
Schlagwörter	Leitfaden; Unterricht; Lehrer; Profiles; Science Laboratories; Genetics; Chemistry; Undergraduate Students; Science Instruction; Teaching Methods + Suchen Sie Ihr Suchwort? Lesson concept; Instruction; Unterrichtsentwurf; Unterrichtsprozess; Teacher; Teachers; Lehrerin; Lehrende; Charakterisierung; Profilanalyse; Humangenetik; Chemie; Teaching of science; Science education; Natural sciences Lessons; Naturwissenschaftlicher Unterricht; Teaching method; Lehrmethode; Unterrichtsmethode
Abstract	Deoxyribonucleic acid (DNA) profiles can be generated by a variety of techniques incorporating different types of DNA markers. Simple methods are commonly utilized in the undergraduate laboratory, but with certain drawbacks. In this article, the author presents an advancement of the "Alu" dimorphism technique involving two tetraplex polymerase chain reaction (PCR) analyses that yield 6,561 possible genotypes. This technique is a simple rapid method of generating complex DNA profiles that can easily be performed in an undergraduate laboratory. Students have an opportunity to analyze their own DNA and incorporate it into a forensic analysis. Additionally, with the same technique, students have the opportunity to assess paternity. Furthermore, students can use the data to determine allele frequencies and perform Hardy-Weinberg analysis. (Contains 5 figures and 1 table.) (ERIC).
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Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2017/4/10