Undergraduate Laboratory Series That Employs a Complete Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Experiment for Determination of a Single Nucleotide Polymorphism in CYP2R1 Gene

Autor/inn/en	Sedky, Nada K.; Arafa, Reem K.
Titel	Undergraduate Laboratory Series That Employs a Complete Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Experiment for Determination of a Single Nucleotide Polymorphism in CYP2R1 Gene
Quelle	In: Biochemistry and Molecular Biology Education, 50 (2022) 2, S.193-200 (8 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Zusatzinformation	ORCID (Arafa, Reem K.)
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	1470-8175
DOI	10.1002/bmb.21604
Schlagwörter	Biochemistry; Science Instruction; Undergraduate Study; College Science; Science Laboratories; Science Process Skills; Genetics; Computer Uses in Education; Conventional Instruction; Science Experiments; Laboratory Experiments + Suchen Sie Ihr Suchwort? Biochemie; Teaching of science; Science education; Natural sciences Lessons; Naturwissenschaftlicher Unterricht; Grundstudium; Humangenetik; Computernutzung; Laboratory work; Laborarbeit
Abstract	Nowadays, novel Biochemistry lab techniques are being introduced at a very fast pace in scientific research. This requires development of new labs for undergraduate Biochemistry courses to equip the students with up-to-date techniques. However, the time limit of Biochemistry labs for undergraduate students represents a major obstacle. This article presents a clear set of laboratory exercises designed to introduce students to the use of polymerase chain reaction-restriction-fragment length polymorphism (PCR-RFLP) as a means of detection of genetic variants. Three consecutive lab experiments have been designed for the undergraduate students to serve this purpose. The first session was performed in a computer lab (dry lab) where students were taught how to obtain a specific gene sequence, identify an exact single nucleotide polymorphism location, choose the target sequence for amplification, design specific primers for this particular sequence and choose the most suitable restriction enzyme from web tools. The second and third lab sessions were performed as wet labs where in the second lab session, students optimized PCR conditions and performed a successful PCR. The PCR products were kept for use in the third lab session where they utilized the selected restriction enzyme and carried out gel electrophoresis to determine the exact genotype. (As Provided).
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Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2024/1/01