Crime Scene Investigation: Simulated Post-Mortem LC-MS/MS Analysis of Cocaine and Cocaine Metabolites in Synthetic Human Serum

Autor/in	Vergne, Matthew J.
Titel	Crime Scene Investigation: Simulated Post-Mortem LC-MS/MS Analysis of Cocaine and Cocaine Metabolites in Synthetic Human Serum
Quelle	In: Journal of Chemical Education, 98 (2021) 11, S.3567-3571 (5 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Zusatzinformation	ORCID (Vergne, Matthew J.)
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	0021-9584
DOI	10.1021/acs.jchemed.1c00161
Schlagwörter	Science Experiments; Science Laboratories; Laboratory Experiments; Undergraduate Students; Chemistry; College Science; Drug Abuse; Cocaine; Metabolism; Spectroscopy; Measurement + Suchen Sie Ihr Suchwort? Laboratory work; Laborarbeit; Chemie; Kokain; Stoffwechsel; Spektroskopie; Messverfahren
Abstract	This laboratory experiment introduces upper-division undergraduate analytical chemistry students to liquid chromatography-tandem mass spectrometry (LC-MS/MS) in a forensic application: the analysis of cocaine and metabolites of cocaine in simulated serum ostensibly from drug overdose victims. For this investigation, students developed the analytical methods. First, the students determined the multiple reaction monitoring (MRM) transitions and optimized collision energy. The MS methods developed were then added to an LC method for LC-MS/MS quantitation with a 2.5 min run time. Students successfully quantified the drugs and metabolites, and they were able to identify which samples indicated cocaine use and overdose. (As Provided).
Anmerkungen	Division of Chemical Education, Inc. and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2024/1/01