Inhibition of Lipase by Orlistat: Kinetics Combined with "In Silico" Approaches to Visualize Interactions

Autor/inn/en	Candela, Maria Fernanda; Arenas, Nelson E.; Caicedo, Obradith; Malagon, Andres
Titel	Inhibition of Lipase by Orlistat: Kinetics Combined with "In Silico" Approaches to Visualize Interactions
Quelle	In: Journal of Chemical Education, 98 (2021) 5, S.1762-1767 (6 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Zusatzinformation	ORCID (Malagon, Andres)
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	0021-9584
DOI	10.1021/acs.jchemed.0c01184
Schlagwörter	Biochemistry; Drug Therapy; Kinetics; Measurement; Inhibition; Simulation; Scientific Concepts; Laboratory Procedures + Suchen Sie Ihr Suchwort? Biochemie; Kinetik; Messverfahren; Hemmung; Simulation program; Simulationsprogramm; Laboruntersuchung
Abstract	The inhibition of porcine pancreatic lipase by the drug orlistat was studied through a series of activities that combine experimental and computational procedures. The enzymatic activity of the lipase was determined by measuring enzyme kinetics and calculating parameters such as K[subscript m] and V[subscript max] from experimental data. Assays of lipase inhibition by orlistat were used to construct Linewaver-Burk and Dixon plots. Docking calculations are highly recommended to visualize the enzyme-ligand interactions and as an approach to decipher the inhibitor mechanism of action. Furthermore, sequence analysis was included to compare enzyme structures across different organisms, specifically between human and pig as closely related study models, and complement the docking simulations. The educational project proposed here for biochemistry or pharmacy students can enhance the understanding of concepts regarding enzyme kinetics, binding sites, and enzyme-ligand interactions through visualizations. (As Provided).
Anmerkungen	Division of Chemical Education, Inc. and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2024/1/01