Using Rat Operant Delayed Match-to-Sample Task to Identify Neural Substrates Recruited with Increased Working Memory Load

Autor/inn/en	Gobin, Christina; Wu, Lizhen; Schwendt, Marek
Titel	Using Rat Operant Delayed Match-to-Sample Task to Identify Neural Substrates Recruited with Increased Working Memory Load
Quelle	In: Learning & Memory, 27 (2020) 11, S.467-476 (10 Seiten) PDF als Volltext Verfügbarkeit
Zusatzinformation	ORCID (Schwendt, Marek)
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	1072-0502
DOI	10.1101/lm.052134.120
Schlagwörter	Animals; Short Term Memory; Brain Hemisphere Functions; Training; Neurological Organization; Cognitive Processes; Difficulty Level + Suchen Sie Ihr Suchwort? Animal; Tier; Tiere; Kurzzeitgedächtnis; Ausbildung; Cognitive process; Kognitiver Prozess; Schwierigkeitsgrad
Abstract	The delayed match-to-sample task (DMS) is used to probe working memory (WM) across species. While the involvement of the PFC in this task has been established, limited information exists regarding the recruitment of broader circuitry, especially under the low- versus high-WM load. We sought to address this question by using a variable-delay operant DMS task. Male Sprague-Dawley rats were trained and tested to determine their baseline WM performance across all (0- to 24-sec) delays. Next, rats were tested in a single DMS test with either 0- or 24-sec fixed delay, to assess low-/high-load WM performance. "c-Fos" mRNA expression was quantified within cortical and subcortical regions and correlated with WM performance. High WM load up-regulated overall "c-Fos" mRNA expression within the PrL, as well as within a subset of mGlu5+ cells, with load-dependent, local activation of protein kinase C (PKC) as the proposed underlying molecular mechanism. The PrL activity negatively correlated with choice accuracy during high load WM performance. A broader circuitry, including several subcortical regions, was found to be activated under low and/or high load conditions. These findings highlight the role of mGlu5- and/or PKC-dependent signaling within the PrL, and corresponding recruitment of subcortical regions during high-load WM performance. (As Provided).
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Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2024/1/01