A Research-Inspired Biochemistry Laboratory Module--Combining Expression, Purification, Crystallization, Structure-Solving, and Characterization of a Flavodoxin-Like Protein

Autor/inn/en	Hammerstad, Marta; Røhr, Åsmund K.; Hersleth, Hans-Petter
Titel	A Research-Inspired Biochemistry Laboratory Module--Combining Expression, Purification, Crystallization, Structure-Solving, and Characterization of a Flavodoxin-Like Protein
Quelle	In: Biochemistry and Molecular Biology Education, 47 (2019) 3, S.318-332 (15 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Zusatzinformation	ORCID (Hersleth, Hans-Petter)
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	1470-8175
DOI	10.1002/bmb.21218
Schlagwörter	Biochemistry; Science Laboratories; Laboratory Training; Science Process Skills; Molecular Biology; Molecular Structure; Genetics; Scientific Research; Student Projects; Active Learning + Suchen Sie Ihr Suchwort? Biochemie; Laborkunde; Molekularbiologie; Humangenetik; Schulprojekt; Aktives Lernen
Abstract	Many laboratory courses consist of short and seemingly unconnected individual laboratory exercises. To increase the course consistency, relevance, and student engagement, we have developed a research-inspired and project-based module, "From Gene to Structure and Function". This 2.5-week full-day biochemistry and structural biology module covers protein expression, purification, structure solving, and characterization. The module is centered around the flavodoxin-like protein NrdI, involved in the activation of the bacterial ribonucleotide reductase enzyme system. Through an in-depth focus on one specific protein, the students will learn the basic laboratory skills needed in order to generate a broader knowledge and breadth within the field. With respect to generic skills, the students report their findings as a scientific article, with the aim to learn to present concise research results and write scientific papers. The current research-inspired project has the potential of being further developed into a more discovery-driven project and extended to include other molecular biological techniques or biochemical/biophysical characterizations. In student evaluations, this research-inspired laboratory course has received very high ratings and been highly appreciated, where the students have gained research experience for more independent future work in the laboratory. (As Provided).
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Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2020/1/01