A Designed Experiment: Polymorphism Analysis of Angiotensin-Converting Enzyme Gene from Human Buccal Epithelial Cells

Autor/inn/en	Jiao, Li; Xueli, Li; Xiujuan, Shi; Song, Jia; Jie, Xu; Lixia, Lv; Wenzhuo, Yang; Lei, Xu
Titel	A Designed Experiment: Polymorphism Analysis of Angiotensin-Converting Enzyme Gene from Human Buccal Epithelial Cells
Quelle	In: Biochemistry and Molecular Biology Education, 47 (2019) 2, S.168-174 (7 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Zusatzinformation	ORCID (Jiao, Li)
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	1470-8175
DOI	10.1002/bmb.21215
Schlagwörter	Biochemistry; Molecular Biology; Science Experiments; Medical Students; Genetics; Hands on Science; Science Instruction; Medical Education; Foreign Countries; China + Suchen Sie Ihr Suchwort? Biochemie; Molekularbiologie; Humangenetik; Teaching of science; Science education; Natural sciences Lessons; Naturwissenschaftlicher Unterricht; Medizinische Ausbildung; Ausland
Abstract	For medical students, we combine the laboratory practice with clinical applications by developing biochemical and molecular biology experiments. In this experiment, students first collect their own buccal epithelial cells by a noninvasive mouthwash method. Then, they extract genomic DNA and perform polymerase chain reaction (PCR) to amplify angiotensin-converting enzyme (ACE) gene using genomic DNA as a template. Finally, the polymorphism of ACE gene is observed by electrophoresis. Students not only learn the techniques but also acquire knowledge of the ACE gene polymorphism. By establishing the relationship among ACE polymorphism and high blood pressure and myocardial hypertrophy, students should be able to understand the gene polymorphism and its association with susceptibility to disease. This laboratory practice teaching can also stimulate desire to do scientific research. Experimental results from many individuals can help us determine and analyze the fractions of ACE gene types in Chinese cohorts. Such an experiment strongly activates students and provides a solid foundation for the medical students' future research and clinical application. (As Provided).
Anmerkungen	Wiley-Blackwell. 350 Main Street, Malden, MA 02148. Tel: 800-835-6770; Tel: 781-388-8598; Fax: 781-388-8232; e-mail: cs-journals@wiley.com; Web site: http://www.wiley.com/WileyCDA
Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2020/1/01