Using the Scratch Assay to Study Cell Migration in an Inquiry-Based Cell Biology Lab

Autor/inn/en	Asmus, Stephen E.; Batey, Kaylind G.; Gale-Butto, Serena N.; Schmitt, Barrie L.
Titel	Using the Scratch Assay to Study Cell Migration in an Inquiry-Based Cell Biology Lab
Quelle	In: Journal of Biological Education, 53 (2019) 2, S.157-164 (8 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	0021-9266
DOI	10.1080/00219266.2018.1447004
Schlagwörter	Science Laboratories; Biology; Cytology; Inquiry; Undergraduate Students; Active Learning; Introductory Courses; Genetics; Biochemistry + Suchen Sie Ihr Suchwort? Biologie; Zytologie; Aktives Lernen; Einführungskurs; Humangenetik; Biochemie
Abstract	Cell migration, a fundamental process in development, wound healing, and immune function, is a common topic in undergraduate cell biology courses. We developed laboratory exercises with an inquiry-based learning (IBL) approach in which cell migration could be examined with the scratch assay, adapted from the primary literature. A narrow scratch was created in a confluent monolayer of cells growing on the bottom of a cell culture dish. Migration into the resultant cell-free zone from both sides of the scratch was measured after one day using the scale bar function of a digital camera. The Chinese hamster ovary cell line was used, but any adherent cell type could be examined. Students used the scratch assay to formulate hypotheses and design experiments in which variables affecting cell migration could be investigated. For example, the effect of cytoskeletal disruption was evaluated by adding the microtubule- and microfilament-disrupting drugs, colcemid and phallacidin, respectively, to the growth medium when the scratch was made. Optimal drug concentration parameters were determined for students to reference. Low drug concentrations inhibited cell migration, while higher concentrations killed the cells. This study demonstrated that the scratch assay is an accessible IBL method for studying cell migration. (As Provided).
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Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2020/1/01