Illustration of Sexual Differentiation to Undergraduates with a Conditional Gene Expression System

Autor/in	Shen, Jie
Titel	Illustration of Sexual Differentiation to Undergraduates with a Conditional Gene Expression System
Quelle	In: Biochemistry and Molecular Biology Education, 46 (2018) 5, S.523-526 (4 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Zusatzinformation	ORCID (Shen, Jie)
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	1470-8175
DOI	10.1002/bmb.21160
Schlagwörter	Leitfaden; Unterricht; Lehrer; Gender Differences; Genetics; Biochemistry; Sex; Laboratory Procedures; Inhibition; Males; Human Body; Molecular Biology; Individual Development; Undergraduate Students + Suchen Sie Ihr Suchwort? Lesson concept; Instruction; Unterrichtsentwurf; Unterrichtsprozess; Teacher; Teachers; Lehrerin; Lehrende; Geschlechterkonflikt; Humangenetik; Biochemie; Geschlecht; Geschlechtsverkehr; Laboruntersuchung; Hemmung; Male; Männliches Geschlecht; Menschlicher Körper; Molekularbiologie; Individuelle Entwicklung
Abstract	This work describes the development and evaluation of a two-session laboratory class based on Tet-on technology and sex determination in the fruit fly "Drosophila melanogaster." The Tet-on system allows conditional control of gene expression, when doxycycline is applied. A laboratory exercise has been developed to illustrate how the Tet-on technology conditionally over-expresses the key sex determining gene transformer (tra) during development and how to inhibit sex differentiation in males, results in a lack of external genitalia and sex comb size reduction. The laboratory practice is inexpensive and straightforward, while allowing students to understand well how molecular biology technology can change biological processes, including development. (As Provided).
Anmerkungen	Wiley-Blackwell. 350 Main Street, Malden, MA 02148. Tel: 800-835-6770; Tel: 781-388-8598; Fax: 781-388-8232; e-mail: cs-journals@wiley.com; Web site: http://www.wiley.com/WileyCDA
Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2020/1/01