The Alcohol Dehydrogenase Kinetics Laboratory: Enhanced Data Analysis and Student-Designed Mini-Projects

Autor/in	Silverstein, Todd P.
Titel	The Alcohol Dehydrogenase Kinetics Laboratory: Enhanced Data Analysis and Student-Designed Mini-Projects
Quelle	In: Journal of Chemical Education, 93 (2016) 5, S.963-970 (8 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	0021-9584
DOI	10.1021/acs.jchemed.5b00610
Schlagwörter	Data Analysis; Student Projects; Kinetics; Biochemistry; Research Design; Thermodynamics; Spectroscopy; Undergraduate Students; Molecular Biology; Teaching Methods; Scientific Methodology; Scientific Principles + Suchen Sie Ihr Suchwort? Auswertung; Schulprojekt; Kinetik; Biochemie; Forschungsdesign; Thermodynamik; Spektroskopie; Molekularbiologie; Teaching method; Lehrmethode; Unterrichtsmethode
Abstract	A highly instructive, wide-ranging laboratory project in which students study the effects of various parameters on the enzymatic activity of alcohol dehydrogenase has been adapted for the upper-division biochemistry and physical biochemistry laboratory. Our two main goals were to provide enhanced data analysis, featuring nonlinear regression, and also to give students experience in experimental design. Students use appropriate kinetic and thermodynamic equations to fit their data from Michaelis-Menten plots, enzyme activity pH profiles, inhibitor and denaturant concentration profiles, and temperature-dependence plots. Experiments at the end of this project are designed and implemented by student pairs, thus preparing them for independent research. (As Provided).
Anmerkungen	Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2020/1/01