Literaturnachweis - Detailanzeige
| Autor/inn/en | Alty, Lisa T.; LaRiviere, Frederick J. |
|---|---|
| Titel | Peptide Mass Fingerprinting of Egg White Proteins |
| Quelle | In: Journal of Chemical Education, 93 (2016) 4, S.772-777 (6 Seiten)Infoseite zur Zeitschrift
PDF als Volltext |
| Sprache | englisch |
| Dokumenttyp | gedruckt; online; Zeitschriftenaufsatz |
| ISSN | 0021-9584 |
| DOI | 10.1021/acs.jchemed.5b00625 |
| Schlagwörter | Leitfaden; Unterricht; Lehrer; Undergraduate Students; Biochemistry; Science Experiments; Molecular Biology; Courseware; Scientific Concepts; Scientific Principles; Web Based Instruction; Spectroscopy; Teaching Methods; Laboratory Experiments; Virginia |
| Abstract | Use of advanced mass spectrometry techniques in the undergraduate setting has burgeoned in the past decade. However, relatively few undergraduate experiments examine the proteomics tools of protein digestion, peptide accurate mass determination, and database searching, also known as peptide mass fingerprinting. In this experiment, biochemistry students digest a protein mixture from egg white using the enzyme trypsin; liquid chromatography electrospray ionization time-of-flight mass spectrometry (LC--ESI-TOF-MS) separates the resulting peptides and determines their accurate masses. Instrument software is used to match these peptides to the sequences of known egg white proteins, obtained from an online source. Students then use online protein database search software to match the peptides to the protein and score the results. (As Provided). |
| Anmerkungen | Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc |
| Erfasst von | ERIC (Education Resources Information Center), Washington, DC |
| Update | 2020/1/01 |
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