Two-Dimensional Nuclear Magnetic Resonance Structure Determination Module for Introductory Biochemistry: Synthesis and Structural Characterization of Lyso-Glycerophospholipids

Autor/inn/en	Garrett, Teresa A.; Rose, Rebecca L.; Bell, Sidney M.
Titel	Two-Dimensional Nuclear Magnetic Resonance Structure Determination Module for Introductory Biochemistry: Synthesis and Structural Characterization of Lyso-Glycerophospholipids
Quelle	In: Biochemistry and Molecular Biology Education, 41 (2013) 2, S.87-94 (8 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	1470-8175
DOI	10.1002/bmb.20668
Schlagwörter	Diagnostic Tests; Science Laboratories; Biochemistry; College Science; Nuclear Energy; Knowledge Level; Science Instruction; Instructional Effectiveness; Learning Modules; Scientific Concepts; Teaching Methods; Molecular Biology; Laboratory Experiments; Laboratory Procedures; Undergraduate Students; Molecular Structure; New York + Suchen Sie Ihr Suchwort? Diagnostic test; Diagnostischer Test; Biochemie; Atomenergie; Kernenergie; Wissensbasis; Teaching of science; Science education; Natural sciences Lessons; Naturwissenschaftlicher Unterricht; Unterrichtserfolg; Learning module; Lernmodul; Teaching method; Lehrmethode; Unterrichtsmethode; Molekularbiologie; Laboratory work; Laborarbeit; Laboruntersuchung
Abstract	In this laboratory module, introductory biochemistry students are exposed to two-dimensional [superscript 1]H-nuclear magnetic resonance of glycerophospholipids (GPLs). Working in groups of three, students enzymatically synthesized and purified a variety of 2-acyl lyso GPLs. The structure of the 2-acyl lyso GPL was verified using [superscript 1]H-correlation spectroscopy. Students scored significantly higher on an assessment of NMR knowledge after having participated in this lab module and in comparison to a similar cohort who did not participate. In addition, student confidence in their NMR knowledge and abilities increased 62% following the module and correlated with their ability to apply their NMR knowledge. Based on these results, the laboratory module was very effective at providing students with a more extensive understanding of the underlying concepts of NMR as a tool for structural determination. (Contains 1 table and 7 figures.) (As Provided).
Anmerkungen	Wiley-Blackwell. 350 Main Street, Malden, MA 02148. Tel: 800-835-6770; Tel: 781-388-8598; Fax: 781-388-8232; e-mail: cs-journals@wiley.com; Web site: http://www.wiley.com/WileyCDA
Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2017/4/10