Apoptosis: A Four-Week Laboratory Investigation for Advanced Molecular and Cellular Biology Students

Autor/inn/en	DiBartolomeis, Susan M.; Mone, James P.
Titel	Apoptosis: A Four-Week Laboratory Investigation for Advanced Molecular and Cellular Biology Students
Quelle	In: Cell Biology Education, 2 (2003) 4, S.275-295 (21 Seiten) PDF als Volltext Verfügbarkeit
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	1536-7509
DOI	10.1187/cbe.03-06-0027
Schlagwörter	Research Design; Oncology; Genetics; Science Instruction; Molecular Biology; Data Interpretation; Laboratory Experiments; Advanced Students; Scientific Research; Undergraduate Students; Cytology; Teaching Methods + Suchen Sie Ihr Suchwort? Forschungsdesign; Onkologie; Humangenetik; Teaching of science; Science education; Natural sciences Lessons; Naturwissenschaftlicher Unterricht; Molekularbiologie; Data evaluation; Datenauswertung; Laboratory work; Laborarbeit; Fortgeschrittener; Zytologie; Teaching method; Lehrmethode; Unterrichtsmethode
Abstract	Over the past decade, apoptosis has emerged as an important field of study central to ongoing research in many diverse fields, from developmental biology to cancer research. Apoptosis proceeds by a highly coordinated series of events that includes enzyme activation, DNA fragmentation, and alterations in plasma membrane permeability. The detection of each of these phenotypic changes is accessible to advanced undergraduate cell and molecular biology students. We describe a 4-week laboratory sequence that integrates cell culture, fluorescence microscopy, DNA isolation and analysis, and western blotting (immunoblotting) to follow apoptosis in cultured human cells. Students working in teams chemically induce apoptosis, and harvest, process, and analyze cells, using their data to determine the order of events during apoptosis. We, as instructors, expose the students to an environment closely simulating what they would encounter in an active cell or molecular biology research laboratory by having students coordinate and perform multiple tasks simultaneously and by having them experience experimental design using current literature, data interpretation, and analysis to answer a single question. Students are assessed by examination of laboratory notebooks for completeness of experimental protocols and analysis of results and for completion of an assignment that includes questions pertaining to data interpretation and apoptosis. (Contains 11 figures and 1 table.) (As Provided).
Anmerkungen	American Society for Cell Biology. 8120 Woodmont Avenue Suite 750, Bethesda, MD 20814-2762. Tel: 301-347-9300; Fax: 301-347-9310; e-mail: ascbinfo@ascb.org; Website: http://www.ascb.org
Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2017/4/10